Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 2

FEBS Letters

Shimomura, Takeshi¹  Kitamura, Naomi²  Denda, Kimitoshi²  Kawaguchi, Toshiya¹  Qin, Li² 

[1] Research Center, Mitsubishi Chemical Corp., Aoba-ku, Yokohama 227, Japan;Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226, Japan

关键词: Hepatocyte growth factor;    Scatter factor;    Hepatocyte growth factor activator;    Hepatocyte growth factor activator inhibitor;    Kunitz domain;   

DOI  :  10.1016/S0014-5793(98)01105-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatocyte growth factor activator inhibitor type 2 (HAI-2) was identified as a potent inhibitor of hepatocyte growth factor activator (HGF activator). The primary translation product of HAI-2 contains two Kunitz domains. To characterize their function, we introduced a point mutation into the reactive site of each Kunitz domain, and assayed the mutants for their HGF activator inhibitory activity. A point mutation in the COOH-terminal Kunitz domain did not affect the activity of HAI-2, whereas a point mutation in the NH₂-terminal Kunitz domain markedly reduced the activity. These results suggest that the NH₂-terminal Kunitz domain is mainly responsible for the HGF activator inhibitory activity of HAI-2.

【 授权许可】

Unknown