Proteolytic processing of the hepatocyte growth factor/scatter factor receptor by furin

FEBS Letters

Kitamura, Naomi¹  Hatsuzawa, Kiyotaka³  Komada, Masayuki¹  Shibamoto, Sayumi⁴  Ito, Fumiaki⁴  Nakayama, Kazuhisa² 

[1] Institute for Liver Research, Kansai Medical University, Moriguchi, Osaka 570, Japan;Institute of Biological Sciences and Gene Experiment Center, University of Tsukuba, Tsukuba, Ibaraki 305, Japan;Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan;Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573-01, Japan

关键词: Hepatocyte growth factor;    Scatter factor;    c-Met receptor;    Proteolytic processing;    Furin;   

DOI  :  10.1016/0014-5793(93)80958-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hepatocyte growth factor/scatter factor (HGF/SF) receptor consists of an α- and a β-subunit, which are derived from a single-chain precursor by endoproteolytic processing. The precursor is not proteolytically processed in LoVo colon carcinoma cells. The uncleaved receptor immunopurified from the cells was cleaved in vitro by furin. Furthermore, the HGF/SF receptor was proteolytically processed in LoVo cells transfected with furin cDNA. These results indicate that furin is a processing endoprotease for the HGF/SF receptor. Tyrosine autophosphorylation of the uncleaved receptor was induced by HGF/SF, and the growth of the cells expressing the uncleaved receptor was stimulated by HGF/SF, indicating that the proteolytic processing of the receptor is not essential for the signal transduction of HGF/SF.

【 授权许可】

Unknown