Suppression of peroxisomal lipid β‐oxidation enzymes by TNF‐α

FEBS Letters

Beier, Konstantin¹  Fahimi, H.Dariush¹  Völkl, Alfred¹ 

[1] Institut für Anatomie und Zellbiologie II, Universiteit Heidelberg, Heidelberg, Germany

关键词: Peroxisome;    Tumor-necrosis-factor;    Immunocytochemistry;    Lipid β-oxidation;    Hyperlipidemia;   

DOI  :  10.1016/0014-5793(92)81347-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

TNF-α is a potent cytokine which induces marked hyperlipidemia. Because of the important role of peroxisomes in lipid metabolism we investigated the effects of human recombinant TNF-α upon rat liver peroxisomal enzymes. Sixteen hours after the administration of a single dose of 25 μg of TNF-α to male rats the activity of peroxisomal fatty acyl-CoA oxidase was reduced by 50%. This was confirmed also by immunoblotting and by quantitative immunoelectron microscopy which in addition revealed substantial reduction of the trifunctional protein (hydratase—dehydrogenase—isomerase) in peroxisomes. These observations suggest that the suppression of peroxisomal β-oxidation may contribute to the perturbation of the lipid metabolism induced by TNF-α.

【 授权许可】

Unknown