Immunocytochemical evidence for the acidic nature of peroxisomes in methylotrophic yeasts

FEBS Letters

Harder, Wim²  Waterham, Hans R.²  Keizer-Gunnink, Ineke²  Veenhuis, Marten³  Goodman, Joel M.¹ 

[1] Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA;Department of Microbiology, Biological Centre, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands;Laboratory for Electron Microscopy, Biological Centre, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands

关键词: Peroxisome;    Proton gradient;    Immunocytochemistry;    (Candida boidinii;    Hansenula polymorpha);   

DOI  :  10.1016/0014-5793(90)80142-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possible acidic nature of the peroxisomal matrix present in intact yeast cells was studied immunocytochemically, using the weak base DAMP as a probe. Spheroplasts of methanol-grown Candida boidinii and Hansenula polymorpha were regenerated and incubated with DAMP. After immunogold labelling, using antibodies against DAMP, a specific accumulation of gold particles was observed on the peroxisomal profiles. This labelling was absent in controls, performed in the presence of ionophores or chloroquine. These results support earlier observations, that in intact cells a pH-gradient exists across the peroxisomal membrane. Experiments, carried out on osmotically swollen spheroplasts indicated that maintenance of this pH-gradient is strongly related to the cell's integrity.

【 授权许可】

Unknown