Over‐expression of the small heat‐shock protein, hsp25, inhibits growth of Ehrlich ascites tumor cells

FEBS Letters

Gaestel, Matthias¹  Bielka, Heinz¹  Knauf, Ursula¹ 

[1] Max Delbrück Center for Molecular Medicine, R-Rössle-Str. 10, Berlin, D1115, Germany

关键词: Heat-shock protein;    Small stress protein;    Transfected cell;    hsp25;    Cell proliferation;    Ehrlich ascites tumor;    EAT;    Ehrlich ascites tumor;    hsp;    heat-shock protein;    hsp25;    murine small heat-shock proteins;    hsp27;    human small heat-shock protein;    EAT 116 and EAT 118;    hsp25 over-expressing Ehrlich ascites tumor cell lines;   

DOI  :  10.1016/0014-5793(92)80793-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

hsp25 is a small, growth-related, mammalian stress protein which is highly accummulated in the stationary phase or Ehrlich ascites tumor in vivo. Ehrlich ascites cells cultivated in vitro under conditions of continuous exponential growth express hsp25 only at a low level. These cells were stably transfected with an eukaryotic expression vector carrying the coding sequence of the small heat-shock protein, hsp25, under control of the murine metallothionein promoter. The resulting cell lines (EAT 116 and EAT 118) exhibit constitutive over-expression of the small heat-shock protein. hsp25, which can be further increased by induction with cadmium. Both cell lines show increased thermoresistance. The in vitro proliferation rate of the transfected cell lines EAT 116 and EAT 118 is significantly decreased depending on the degree of cadmium-regulated over-expression of hsp25. Furthermore, a significant delay in Ehrlich ascites tumor growth in mice using the hsp25 over-expressing cells for primary inoculation could be demonstrated.

【 授权许可】

Unknown