TATA box‐independent transcription of the human tissue plasminogen activator gene initiates within a sequence conserved in related genes

FEBS Letters

Henderson, Beric R¹  Sleigh, Merilyn J.² 

[1] Medical Oncology Unit, Department of Medicine, University of Sydney, Westmead Hospital, Westmead NSW 2145, Australia;Commonwealth Scientific and Industrial Research Organisation (CSIRO), Division of Biomedical Engineering, 103 Delhi Road, North Ryde, NSW 2113, Australia

关键词: Tissue plasminogen activator;    Transcription;    TATA-box;    mRNA;    bp;    base pair;    CAT;    chloramphenicol acetyl transferase;    cDNA;    complementary DNA;    DME;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    PMA;    phorbol-12-myristate-13-acetate;    tPA;    tissue-type plasminogen activator;   

DOI  :  10.1016/0014-5793(92)81080-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transcription of the human tissue-type plasminogen activator (tPA) gene has been reported to initiate from a single site proximal to a TATA box motif (1985, J. Biol. Chem. 260, 11223–11230). In this study, we utilized primer extension analysis to evaluate the tPA mRNA start site in phorbol-12-myristate 13-acetate (PMA) induced WI-38 man lung fibroblast cells. Whilst some tPA mRNA initiated from the predicted TATA-proximal location (+1), a 10-fold greater proportion of tPA mRNA transcripts initiated 110 bases downstream from a sequence conserved and utilized as the TATA-independent transcription start site in the rodent tPA genes. Moreover, the transfection and expression in different cell types of a cosmid containing the entire human tPA gene resulted in utilization of the same downstream (+110) start site. We propose that this, rather than the previously published position, is the major transcriptional initiation point for the human tPA gene. A core sequence (5′-CAGAGCTG-3′) was identified which is common to the TATA-independent mRNA start sites of the human, mouse and rat tPA genes, and which demonstrates only partial similarity to sequences found at the initiation point of other TATA-independent genes.

【 授权许可】

Unknown