Over‐expression of human phospholipase C‐γ2 enhances platelet‐derived growth factor‐induced mobilization of intracellular Ca2+ and the release of arachidonic acid and prostaglandins in NIH 3T3 fibroblasts

FEBS Letters

Totzke, Frank¹  Dieter, Peter¹  Hug, Hubert¹  Marmé, Dieter¹  Fitzke, Edith¹ 

[1] University of Freiburg, Institute of Molecular Cell Biology, c/o Gödecke AG, Mooswaldallee 1-9, D-7800 Freiburg, Germany

关键词: Phospholipase C-γ2;    Over-expression;    Arachidonic acid;    Prostaglandin: Ca2+ release;    hPLC-γ2;    human phospholipase C-γ2;    PDGF BB;    platelet-derived growth factor B-chain homodimer;    PKC;    protein kinase C;    AA;    arachidonic acid;    PG;    prostaglandin;    PMA;    phorbol 12-myristate 13-acetate;    BSA;    bovine serum albumin;    MEM;    minimum essential medium;    HPLC;    high-performance liquid chromatography;    [Ca2+];    intracellular concentration of free Ca2+;   

DOI  :  10.1016/0014-5793(92)81258-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Over-expression of human phospholipase C-γ2 in murine NIH 3T3 fibroblasis has been shown to result in an increased platelet-derived growth factor-mediated formation of inositol phosphates. Here we show that phospholipase C-γ2 over-expression is associated with an increased platelet-derived growth factor-mediated release of arachidonic acid, prostaglandin E₂, 6-keto prostaglandin F₁₀ and prostaglandin F₂₀. The phorbol ester, calcium ionophore- and fluoride-induced release of arachidonate and its metabolites is not affected by phospholipase C-γ2 over-expression. Over-expression of phospholipase C-γ2 is also associated with an enhancement of platelet-derived growth factor-induced change in intracellular Ca²⁺. These results demonstrate that stimulation of recombinant human phospholipase C-γ2 induces a change in the intracellular Ca²⁺ concentration, a release of arachidonic acid and formation of prostaglandins in NIH 3T3 cells. In control cells platelet-derived growth factor-induced activation of arachidonic acid cascade is rate-limited by the endogenous phospholipase C.

【 授权许可】

Unknown