FEBS Letters | |
Induction of an electrogenic transfer of monovalent cations (K+, NH+ 4) in thylakoid membranes by N,N′‐dicyclohexylcarbodiimide | |
Gubanova, O.N.1  Yaguzhinsky, L.S.1  Red'ko, T.P.1  Opanasenko, V.K.1  | |
[1] Soil Science and Photosynthesis Institute of the Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia | |
关键词: N; N′-Dicyclohexylcarbodiimide; Thylakoid membrane; Electron transport; K+; NH+ 4; Cation transfer; | |
DOI : 10.1016/0014-5793(92)80695-D | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The effect of N,N′-dicyclohexylcarbodiimide (DCCD) on photosynthetic electron transport and light-induced NH+ 4 and K+ uptake in the presence of ammonium or nigericin was studied. DCCD alone had no effect on either the electron transport or the uptake of protons. The simultaneous action of DCCD and low concentrations of ammonium or nigericin was shown to lead to a significant increase in the electron transport rate and a decrease in the steady-state uptake value of H+ and NH+ 4 or K+. The effect of DCCD on these processes was compared with the effect of the ionophore, valinomycin, which transports potassium and ammonium cations through membranes. The conclusion was made that 1.0–1.5 mol DCCD per mol chlorophyll activated the transfer system of monovalent cations (K+ and NH+ 4) in thylakoid membranes.
【 授权许可】
Unknown
【 预 览 】
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