Computational approach towards the three‐dimensional structure of E. coli tyrosine aminotransferase

FEBS Letters

Jäger, J.¹  Solmajer, T.¹  Jansonius, J.N.¹ 

[1] Biozentrum der Universität Basel, Klingelbergstr. 70, CH-4056 Basel, Switzerland

关键词: Tyrosine aminotransferase;    Computer modelling;    X-ray Structure;    Aspartate aminotransferase;    Mutant enzyme;    E. coli;    mAspAT;    mitochondrial aspartate aminotransferase;    eTyrAT;    E. coli tyrosine aminotransferase;    cAspAT;    cytosolic aspartate aminotransferase;    cAspAT;    E. coli aspartate aminotransferase;    PLP;    pyridoxal-5′-phosphate;    PMP;    pyridoxamine-5′-phosphate;    r.m.s;    root mean square;   

DOI  :  10.1016/0014-5793(92)81007-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We present a new model for E. coli tyrosine aminotransferase based on the X-ray structures of the wild type and Val³⁹Leu mutant of E. coli aspartate aminotransferase and computer simulation studies. Active site characteristics of the model are correlated with experimental observations on the specificity of these enzymes towards aromatic/dicarboxylic acid substrates.

【 授权许可】

Unknown