Assignment of the redox potentials to the four haems in Desulfovibrio vulgaris cytochrome c 3 by 2D‐NMR

FEBS Letters

LeGall, Jean¹  Turner, David L.³  Santos, Helena²  Salgueiro, Carlos A.²  Xavier, António V.² 

[1] Department of Biochemistry, University of Georgia, Athens, Georgia 30602, USA;Centro de Technologia Quimica e Biológica and Universidade Nova de Lisboa, Apt. 127, 2780 Oeiras, Portugal;Department of Chemistry, University of Southampton, Southampton SO9 5NH, UK

关键词: Cytochrome c 3;    Multihaem protein;    2D-NMR;    ROESY;    NOESY;    X-ray Structure;    c3DvMF;    Desulfovibrio vulgaris (Miyazaki F.) cytochrome c 3;    c3DvH;    Desulfovibrio vulgaris (Hildenborough) cytochrome c 3;    c3DbN;    Desulfovibrio baculatus (Norway) cytochrome c 3;    ROESY;    Rotating Frame NOE Spectroscopy;    NOESY;    NOE Spectroscopy;    Mi;    haem methyl groups (i = 21;    71;    121;    181) according to the IUPAC-IUB nomenclature;   

DOI  :  10.1016/0014-5793(92)80963-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using 2D-NMR the four haems of Desulfovibrio vulgaris (Hildenborough) cytochromes, within the X-ray structure were fully cross-assigned according to their redox potential. The strategy used was based on a complete network of chemical exchange connectivities between the NMR signals obtained for all oxidation levels to the corresponding ones in the fully reduced spectrum [1992, Eur. J. Biochem., in press]. This unequivocal cross-assignment disagrees within earlier results obtained for the similar protein from Desulfovibrio vulgaris (Miyazaki F.) [1991, FEBS Lett. 285, 149–151]

【 授权许可】

Unknown