Full assignment of heme redox potentials of cytochrome c 3 of D. vulgaris Miyazaki F by 1H‐NMR

FEBS Letters

Akutsu, Hideo¹  Kano, Katsuhiro¹  Park, Jang-Su¹  Niki, Katsumi¹ 

[1] Department of Physical Chemistry, Faculty of Engineering, Yokohama National University, Hodogaya-ku, Yokohama 240, Japan

关键词: Cytochrome c 3;    Tetraheme protein;    Redox potentials of a tetraheme protein;    1H-NMR;    Assignment of redox potential;   

DOI  :  10.1016/0014-5793(91)80746-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Site-specific heme assignment of the ¹H-NMR spectrum of cytochrome c ₃ of D. vulgaris Miyazaki F, a tetraheme protein, was established. The major reduction of the heme turned out to take place in the order of hemes I, III, IV and II (numbering in the crystal structure). The hemes with the smallest and greatest solvent accessibility were reduced at the highest and lowest potentials on average, respectively. A cooperative interheme interaction was attributed to a pair of the closest hemes, namely, hemes III and IV. This assignment can provide the physicochemical basis for the elucidation of electron transfer of this protein.

【 授权许可】

Unknown