mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

FEBS Letters

de Leeuw, Wiljo J.F.¹  Slagboom, P.Eline¹  Vijg, Jan¹ 

[1] Department of Molecular Biology, TNO Institute for Experimental Gerontology, P.O. Box 5815, 2280 HV Rijswijk, The Netherlands

关键词: Tyrosine aminotransferase;    Aging;    DNA methylation;    mRNA;    kb;    kilobasepairs;    OTC;    ornithine transcarbamoylase (EC 2.1.3.3);    TAT;    tyrosine aminotransferase (EC 2.6.1.5);    5mC;    5-methylcytosine;   

DOI  :  10.1016/0014-5793(90)81136-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.

【 授权许可】

Unknown