3′‐Mercapto‐2′,3′‐dideoxynucleotides are high effective terminators of DNA synthesis catalyzed by HIV reverse transcriptase

FEBS Letters

Yuzhakov, Alexander A.¹  Beabealashvilli, Robert Sh.¹  Chidgeavadze, Zurab G.¹ 

[1] Cardiology Research Centre, Academy of Medical Sciences of Russia, Moscow 121552, Russia

关键词: DNA polymerase;    Reverse transcriptase;    Virus;    HIV;    Nucleotide;    Analogue;    Chain termination;   

DOI  :  10.1016/0014-5793(92)80996-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four 3′-mercapto-2′,3′-dideoxynucleoside 5′-triphosphates (A, G, C and T) were tested as DNA chain terminator substrates for calf thymus α-DNA polymerase, E. coli DNA polymerase I Klenow fragment, terminal deoxynucleotidyl transferase and reverse transcriptases of AMV, HIV and MLV viruses. It was shown that the analogues selectively and irreversibly terminated DNA chain elongation by AMV and HIV reverse transcriptases and the terminal transferase. Other DNA polymerases tested did not use the nucleotide analogues as chain terminator substrate.

【 授权许可】

Unknown