A 17.6 kbp region located upstream of the rabbit WAP gene directs high level expression of a functional human protein variant in transgenic mouse milk

FEBS Letters

Bischoff, Rainer²  Degryse, Eric²  Pavirani, Andrea²  Ali-Hadji, Dalila²  Dalemans, Wilfried²  Houdebine, Louis-Marie¹  Perraud, Frédéric²  Thépot, Dominique¹  Devinoy, Eve¹ 

[1] Unité de Differenciation Cellulaire, I.N.R.A., 78350 Jouy-en-Josas, France;TRANSGENE S.A., 11 Rue de Molsheim, 67082 Strasbourg Cédex, France

关键词: Rabbit;    WAP gene;    α1-Antitrypsin;    Transgenic mouse;    Milk;    Plasma kallikrein;   

DOI  :  10.1016/0014-5793(92)80683-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated whether DNA regions present in the rabbit whey acidic protein (WAP) promoter/5′ flanking sequence could potentially confer. in vivo, high level expression or reporter genes. Transgenic mice were generated expressing a variant of human α₁-antitrypsin, which has inhibitory activity against plasma kallikrein under the control of a 17.6 kbp DNA fragment located upstream of the rabbit WAP gene. Up to 10 mg/ml of active and correctly processed recombinant protein were detected in mouse milk, thus suggesting that the far upstream DNA sequences from the rabbit WAP gene might be useful for engineering efficient protein production in the mammary glands or transgenic animals.

【 授权许可】

Unknown