Xenopus oocytes can synthesise but do not secrete the Z variant of human α1‐antitrypsin

FEBS Letters

Foreman, R.C.²  Colman, A.¹  Judah, J.D.² 

[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England;Department of Experimental Pathology, University College London, University Street, London WC1E 6JJ, England

关键词: α1-Antitrypsin;    Human liver mRNA;    Xenopus oocyte;    α1-Antitrypsin secretion PiM variant;    PiZ variant;   

DOI  :  10.1016/0014-5793(84)80211-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human liver mRNA was prepared from a patient homozygous for α₁-antitrypsin deficiency (PiZZ) and from a normal subject (PiMM). Both liver RNAs were microinjected into Xenopus oocytes and α₁-antitrypsin identified by immunoprecipitation. The normal M variant of α₁-antitrypsin is synthesised and secreted by Xenopus oocytes, the abnormal Z protein is not secreted and an intracellular form accumulates in the oocytes. In the presence of tunicamycin an unglycosylated form of M α₁-antitrypsin appears in the incubation medium but no corresponding unglycosylated version of the Z protein is secreted.

【 授权许可】

Unknown