Properties of α1‐antitrypsin secreted by human adenocarcinoma cell lines

FEBS Letters

Seguchi, Kohji¹  Koono, Masashi¹  Kataoka, Hiroaki¹  Inoue, Teruhiko¹ 

[1] Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-16, Japan

关键词: α1-Antitrypsin;    Adenocarcinoma cell;    Glycosylation;   

DOI  :  10.1016/0014-5793(93)80946-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α₁-Antitrypsin; (α₁-AT) produced by various human carcinoma (non-hepatoma) cell lines were analyzed. Five out of eight cell lines secreted detectable amounts of α₁-AT into the conditioned media. All were adenocarcinoma cell lines. The tumor cell-derived α₁-ATs had higher molecular weights (MW) than the normal plasma form. Most of this difference was an overall reflection of altered N-glycosylation. As judged by binding of lectins, the glycosylation had shifted towards higher levels of triantennary oligosaccharides and higher levels of fucosylation. The conditioned media also contained lower MW α₁-AT species, possibly, proteolytically cleaved forms.

【 授权许可】

Unknown