The Euglena gracilis rbcS gene contains introns with unusual borders

FEBS Letters

Chan, Raquel L.¹  Imbault, Patrice¹  Tessier, Luc-Henri¹  Weil, Jacques-Henry¹  Keller, Mario¹ 

[1] Institut de Biologie Moleculaire des Plantes du CNRS, Université Louis Pasteur de Strasbourg, 12 rue du Génŕal Zimmer, 67084 Strasbourg Cedex, France

关键词: Euglena gracilis;    rbcS gene;    Intron;    GT-AG rule;    Splicing;   

DOI  :  10.1016/0014-5793(92)80631-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently shown that, in Euglena gracilis, leader sequences are transferred by trans-splicing to the vast majority of cytoplasmic mRNAs. Trans-splicing is involved in the maturation of the rbcS transcript, which encodes eight small subunits of the ribulose 1,5 bisphosphate carboxylase/oxygenase. In this report, we show that the Euglena rbcS gene introns are different from introns found in plant rbcS genes. In addition these introns do not have the conserved 5′ and 3′ border sequences found in introns of eucaryotic nuclear-encoded pre-mRNAs, and they do not present any homology with self-splicing introns of groups I and II. Secondary structure analyses show that the 5′ and 3′ ends of Euglena introns can base-pair, suggesting that an unusual splicing mechanism exists in Euglena.

【 授权许可】

Unknown