Different flexibility of the upstream regulatory regions of two differently expressed pea rbcS genes studied by theoretical evaluation of DNA distortion energy and cyclization kinetics

FEBS Letters

Savino, Maria¹  De Santis, Pasquale²  Cacchione, Stefano¹ 

[1] Dipartimento di Genetica e Biologia Molecolare, Università di Roma ‘La Sapienza’, P. le A. Moro 5, Roma, Italy;Dipartimento di Chimica, Università di Roma ‘La Sapienza’, P. le A. Moro 5, Roma, Italy

关键词: Sequence dependent DNA superstructure;    DNA distortion energy calculation;    Cyclization kinetics;    rbcS gene;   

DOI  :  10.1016/0014-5793(93)80823-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Different superstructural features of the upstream regulatory regions of the two pea genes rbcS-3A and rbcS-E9 have been derived using a theoretical method, developed in our laboratory a few years ago, which evaluates the DNA distortion energy from a matrix of the deviations of the 16 possible dinucleotides from the standard B conformation. The theoretical analysis, which predicts different flexibilities of the regulatory regions of the two genes, is in satisfactorily good agreement with experimental evaluations from gel electrophoretic mobility and cyclization kinetics, suggesting a possible model to explain the largely different transcription efficiencies of the two genes.

【 授权许可】

Unknown