Membrane‐bound apolipoprotein B is exposed at the cytosolic surface of liver microsomes

FEBS Letters

Bowyer, D.E.²  Groot, P.H.E.⁴  Gherardi, E.¹  Wilkinson, Jane³  Higgins, Joan A.³ 

[1] ICRF Cell Interactions Laboratory, Department of Medicine, Addenbrookes Hospital Cambridge, Cambridge, UK;Department of Pathology, University of Cambridge, Cambridge, UK;Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK;Smith Kline Beecham Research Laboratories, The Frythe, Welwyn, UK

关键词: Apolipoprotein B;    Rabbit;    Liver;    Endoplasmic reticulum membrane;    Topography;    Monoclonal antibody;   

DOI  :  10.1016/0014-5793(92)80581-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used a competitive enzyme-linked immunoassay with a panel of monoclonal antibodies to probe the topography of the membrane-bound form of apolipoprotein B (apo B) in rabbit microsomes. All epitopes investigated were found to be expressed at the cytosolic side of the microsomal membrane under conditions in which the vesicles remained sealed. These results indicate that the membrane-associated form of apolipoprotein B is either at the cytosolic side of the endoplasmic reticulum membrane or integrated into the membrane. From this site apo B may be translocated to the lumen for assembly into VLDL or may be degraded.

【 授权许可】

Unknown