Protein kinase C activity affects glucose‐induced oscillations in cytoplasmic free Ca2+ in the pancreatic B‐cell

FEBS Letters

Köhler, Martin¹  Rorsman, Patrik¹  Berggren, Per-Olof¹  Kindmark, Henrik¹  Larsson, Olof¹  Efendić, Suad¹ 

[1] The Rolf Luft Center for Diabetes Research, Department of Endocrinilogy, Karolinska Institute, Box 60 500, Karolinska Hospital, S-104 01 Stockholm, Sweden

关键词: Pancreatic B-cell;    Stimulus-secretion coupling;    Ca2+-oscillation;    Voltage-dependent Ca2-channel;    Phospholipase C-system;   

DOI  :  10.1016/0014-5793(92)80483-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acute stimulation of protein kinase C (PKC) inhibited glucose-induced slow oscillations in cytoplasmic free Ca²⁺-concentration, [Ca²⁺]_i, in mouse pancreatic B-cells. In PKC-depleted cells glucose induced rapid transients in [Ca²⁺]_i, lasting for approximately 10 s, superimposed on the slow oscillations in [Ca²⁺]_i. It was demonstrated that the transients did not occur in the absence of extracellular Ca²⁺. Each transient typically was preceded by a slow increase in [Ca²⁺]_i, representing the rising phase of an ordinary glucose-induced slow oscillation, and the [Ca²⁺]_i, immediately after a transient was lower than just before the spike. These data further emphasize the interplay between voltage-dependent Ca²⁺-channels and the phospholipase C system in the regulation of B-cell [Ca²⁺]_i-oscillations.

【 授权许可】

Unknown