Absence of insulinotropic glucagon‐like peptide‐I(7–37) receptors on isolated rat liver hepatocytes

FEBS Letters

Exton, John H.³  Mojsov, Svetlana²  Blackmore, Peter F.¹  Habener, Joel F.³ 

[1] Department of Pharmacology, Eastern Virginia Medical School, P.O. Box 1980, Norfolk, VA 23501, USA;Massachusetts General Hospital, Laboratory of Molecular Endocrinology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02114, USA;Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Room 831 Rudolph Light Hall, Nashville, TN 37232, USA

关键词: Glucagon-like peptide-I;    Hepatocyte receptor;    Pancreatic B-cell;    Insulinotropic;    Cyclic AMP;   

DOI  :  10.1016/0014-5793(91)80541-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of glucagon and the glucagon-like peptide GLP-I(7–37) were compared in rat liver hepatocytes. Glucagon elevated cAMP, elevated intracellular free calcium ([Ca²⁺]₁), activated phosphorylase and stimulated gluconeogenesis, whereas GLP-I(7–37) was without effect on any of these parameters. GLP-I(7–37) did not block any of the actions of glucagon. The glucagon analog, des His¹[Glu⁹] glucagon, amide, was a partial agonist in liver, but also was an effective antagonist of glucagon actions in liver but not those of GLP-I(7–37) in islet B cells. It was concluded that in the rat, GLP-I(7–37) is a potent insulin secretagogue [1] but is without effect on liver.

【 授权许可】

Unknown