Nucleotide sequence of a cDNA encoding human tumor necrosis factor β from B lymphoblastoid cell RPMI 1788

FEBS Letters

Endo, Tohru¹  Tsukii, Yuji¹  Okawa, Noriyuki¹  Matsuyama, Noriko¹  Kaji, Akira² 

[1] Tsumura Research Institute for Molecular Genetics, Tsumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-11, Japan;Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA

关键词: cDNA cloning;    Tumor necrosis factor β;    RPMI 1788 cell;    Polymerase chain reaction;   

DOI  :  10.1016/0014-5793(92)80425-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Published sequences of cDNA for human tumor necrosis factor β (TNF-β) have a discrepancy within the coding region as well as exon 1. To resolve these discrepancies we have re-isolated TNF-β cDNA from the human B cell lymphoblastoid cell line, RPMI 1788, and determined its DNA sequence. Results indicate that amino acid 26 is threonine (Thr) instead of asparigine (Asn). In contrast to published sequences, the sequence of the exon 1 region corresponded to the genomic sequence of TNF-β. From our studies we conclude that the TNF-β gene of the human B cell lymphoblastoid cell line, RPMI 1788, is homologous with respect to the TNF-β gene.

【 授权许可】

Unknown