Genomic structure of the gene for copper/zinc‐superoxide dismutase in rice

FEBS Letters

Tanaka, Kunisuke¹  Sakamoto, Atsushi¹  Ohsuga, Hiroyuki¹  Okumura, Takekazu¹ 

[1] Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto 606, Japan

关键词: Active oxygen;    Exon/intron;    Genomic DNA sequence;    SOD gene;    Superoxide dismutase;    Rice (Oryza sativa L.);    SDS;    sodium dodecyl sulfate;    SOD;    superoxide dismutase;    SSC;    150 mM NaCl/15 mM trisodium citrate;    5′ exon;    5′ non-coding exon;    5′ intron;    5′ non-coding intron;   

DOI  :  10.1016/0014-5793(92)81244-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated and determined the nucleotide sequence of the structural gene (sodB) coding for one of the copper/zinc-superoxide dismutase (Cu/Zn-SOD) isozymes from rice plants (Oryza sativa L.). The sodB gene is split into 8 exons spread about 2 kb of the chromosomal DNA, with the coding sequence beginning in the 2nd and ending in the 8th. Although none of sodB introns are inserted into similar positions in Cu/Zn-SOD genes from other sources, four of six introns in the protein-coding region are located at discriminating positions within structural domains of the protein. Genomic Southern analysis indicated that cytosolic Cu/Zn-SOD is coded for by 2–3 genes in the rice genome.

【 授权许可】

Unknown