Messenger RNA sorting in enterocytes Co‐localization with encoded proteins

FEBS Letters

Büller, Hans A.²  Grand, Richard J.³  Moorman, Antoon F.M.¹  Rings, Edmond H.H.M.²  de Boer, Piet A.J.¹  Lamers, Wouter H.¹  Charles, Robert¹  Montgomery, Robert K.³ 

[1] Center for Liver and Intestinal Research (CLDO), Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands;Center for Liver and Intestinal Research (CLDO), Division of Pediatric Gastroenterology and Nutrition G8-260, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands;Center for Liver and Intestinal Research (CLDO), Division of Pediatric Gastroenterology and Nutrition, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA

关键词: Carbamoylphosphate synthetase;    Lactase-phlorizin hydrolase;    Phosphoenolpyruvate carboxykinase;   

DOI  :  10.1016/0014-5793(92)80192-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study describes the intracellular compartmentalization of three different mRNAs in the polarized rat fetal enterocyte. They encode proteins that are known to be localized within different regions of the epithelial cell namely (i) the apical, membrane-bound glycoprotein, lactase-phlorizin hydrolase (lactase), (ii) the mitochondrially localized enzyme, carbamoylphosphate synthetase (CPS), and (iii) the cytoplasmically localized enzyme, phosphoenolpyruvate carboxykinase (PEPCK). These mRNAs are found in close proximity to their respective protein products, i.e. the apical membrane, mitochondria and cytoplasm, respectively. The significance or these observations is twofold: (i) they indicate that mRNAs are sorted into specific domains of the cytosol of intestinal epithelial cells: and (ii) they imply the presence or two distinct pathways of mRNA targeting one that allows transport of mRNAs that are translated on ribosomes associated with the rough endoplasmic reticulum (lactase mRNA), and the other that allows sorting of mRNAs that are translated on free polysames (CPS and PEPCK mRNA).

【 授权许可】

Unknown