Diet‐ and hormone‐induced reversal of the carbamoylphosphate synthetase mRNA gradient in the rat liver lobulus

FEBS Letters

Moorman, Antoon F.M.¹  de Boer, Piet A.J.¹  Lamers, Wouter H.¹  Charles, Robert¹ 

[1] Department of Anatomy and Embryology, University of Amsterdam, AMC, 1105 AZ Amsterdam, The Netherlands

关键词: Carbamoylphosphate synthetase;    Phosphoenolpyruvate carboxykinase;    Glutamine synthetase;    Enzymic zonation;    Hybridization;    in situ;    Rat liver;   

DOI  :  10.1016/0014-5793(90)80494-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A hybridocytochemical analysis of adult liver from normal control and from hormonally and dietary-treated rats was carried out, using radioactively-labelled probes for the mRNAs of glutamine synthetase (GS), carbamoylphosphate synthetase (CPS) and phosphoenolpyruvate carboxykinase (PEPCK). In line with previous findings, GS mRNA is exclusively expressed in a small pericentral compartment, CPS mRNA exclusively in a contiguous large periportal compartment and PEPCK mRNA across the entire porto-central distance. The density of labelling in CPS and PEPCK mRNA-positive hepatocytes decreases in a porto-central direction. Starvation resulted in a reversal of the gradient of CPS mRNA within its periportal compartment; glucose refeeding counteracted this effect. Livers of glucocorticosteroid-treated, starved or diabetic rats also revealed a reversal of the normal gradient of CPS mRNA, but now across the entire porto-central distance. The patterns of expression of GS and PEPCK mRNA remained essentially unchanged, notwithstanding substantial changes in the levels of expression. It is concluded that blood-borne factors constitute the major determinants for the expression patterns of CPS mRNA within the context of the architecture of the liver lobulus.

【 授权许可】

Unknown