FEBS Letters | |
Diet‐ and hormone‐induced reversal of the carbamoylphosphate synthetase mRNA gradient in the rat liver lobulus | |
Moorman, Antoon F.M.1  de Boer, Piet A.J.1  Lamers, Wouter H.1  Charles, Robert1  | |
[1] Department of Anatomy and Embryology, University of Amsterdam, AMC, 1105 AZ Amsterdam, The Netherlands | |
关键词: Carbamoylphosphate synthetase; Phosphoenolpyruvate carboxykinase; Glutamine synthetase; Enzymic zonation; Hybridization; in situ; Rat liver; | |
DOI : 10.1016/0014-5793(90)80494-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A hybridocytochemical analysis of adult liver from normal control and from hormonally and dietary-treated rats was carried out, using radioactively-labelled probes for the mRNAs of glutamine synthetase (GS), carbamoylphosphate synthetase (CPS) and phosphoenolpyruvate carboxykinase (PEPCK). In line with previous findings, GS mRNA is exclusively expressed in a small pericentral compartment, CPS mRNA exclusively in a contiguous large periportal compartment and PEPCK mRNA across the entire porto-central distance. The density of labelling in CPS and PEPCK mRNA-positive hepatocytes decreases in a porto-central direction. Starvation resulted in a reversal of the gradient of CPS mRNA within its periportal compartment; glucose refeeding counteracted this effect. Livers of glucocorticosteroid-treated, starved or diabetic rats also revealed a reversal of the normal gradient of CPS mRNA, but now across the entire porto-central distance. The patterns of expression of GS and PEPCK mRNA remained essentially unchanged, notwithstanding substantial changes in the levels of expression. It is concluded that blood-borne factors constitute the major determinants for the expression patterns of CPS mRNA within the context of the architecture of the liver lobulus.
【 授权许可】
Unknown
【 预 览 】
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