Peroxisomal protein import In vivo evidence for a novel ranslocation competent compartment

FEBS Letters

Heinemann, Petra¹  Just, Wilhelm W.¹ 

[1] Institut für Biochemie I, Universität Heidelberg, Im Neuenheimier Feld 328, D-6900 Heidelberg, Germany

关键词: Hepatocyte;    Peroxisome;    Peroxisomal protein import;    Acyl-CoA oxidase;    SDS-PAGE;    sodium dodecylsulfate polyacrylamide gel electrophoresis;    PMP;    peroxisomal membrane polypeptide;   

DOI  :  10.1016/0014-5793(92)80191-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In homogenates of isolated hepatocytes separated by Nycodenz density gradient centrifugation, two peroxisomal populations are identified that differ in buoyant density. Organelles present in a high density fraction (1.22–1.23 g/cm³) represent mature peroxisomes. Vesicles of intermediate density (1.16–1.17 gm̧³) represent mature peroxisomes. Vesicles of intermediate density (1.16–1.17 g/cm³) are present in much lower concentration and seem to play a particular role in the import and distribution or newly synthesized peroxisomal proteins. In a typical pulse-chase experiment with a 7.5 min pulse, peroxisomal acyl-CoA oxidase is first imported into the peroxisomal fraction of intermediate density. After a chase of up to 30 min, the enzyme is found in mature peroxisomes.

【 授权许可】

Unknown