Growth factor‐stimulated trans plasma membrane electron transport in HL‐60 cells

FEBS Letters

Alcain, Francisco J.²  Navas, Placido²  Morré, D.James¹  Burón, Isabel²  Villalba, Jose M.²  Rodríquez-Aguilera, Juan-Carlos³  Morré, Dorothy M.³ 

[1] Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA;Departmento de Biologia Cellular, Facultad de Ciencias, University of Córdoba, Córdoba, Spain;Departments of Foods and Nutrition, West Lafayette, IN 47907, USA

关键词: Plasma membrane;    Electron transport;    Ascorbate;    A1;    ascorbate radical;    EGF;    epidermal growth factor;    Fe2TF;    diferric transferrin;    WGA;    wheat germ agglutinin;   

DOI  :  10.1016/0014-5793(92)80119-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Electron flow across the plasma membrane of living cells and its rapid modulation by growth factors has been measured continuously through a simple assay procedure whereby the transported electrons are captured by ascorbate free radical to slow the rate of chemical oxidation of ascorbate. The assay provides a direct demonstration of electron transport to an external electron acceptor that is both physiological and impermeant. The reduction of external ascorbate free radical is stimulated by the growth factors, EGF and transform, and is inhibited by wheat germn agglutinin. The results demonstrate, under physiological conditions, the operation of a growth factor- and lecitin-responsive electron transport system at the cell surface using a cultured human cell line.

【 授权许可】

Unknown