Metabotropic responses to acetylcholine and serotonin of Xenopus oocytes injected with rat brain mRNA are transduced by different G‐protein subtypes

FEBS Letters

Takahashi, Hideki¹  Satoh, Masamichi¹  Kaneko, Shuji¹ 

[1] Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606-01, Japan

关键词: GTP-binding protein;    Muscarinic receptor;    5-HTIC receptor;    Phospholipase C;    Antisense DNA;    Xenopus oocyte;   

DOI  :  10.1016/0014-5793(92)80242-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To assign the GTP-binding protein (G-protein) subtype involved in the signal transduction from exogenous receptors to phospholipase C in the Xenopus oocyte translation system, antisense DNA complementary to rat G-protein α-subunit MRNA was designed and injected together with rat brain poly(A)⁺ RNA. Current response of mRNA-injected oocytes to acetylcholine (ACh) was suppressed dose-dependently by a co-injection of G_ilα-antisense DNA, but response of the same oocytes to serotonin (5-HT) was not inhibited. In the oocytes co-injected with G_oα-antisense DNA, the 5-HT response was more effectively suppressed than the ACh response. These results suggest that G_oα but not G_ilα intermediates brain 5-HT_IC receptor function, and in contrast, muscarinic receptors derived from rat brain utilize G_ilα rather than G_oα to activate phospholipase C.

【 授权许可】

Unknown