Coexistence of two calsequestrin isoforms in rabbit slow‐twitch skeletal muscle fibers

FEBS Letters

Margreth, Alfredo¹  Damiani, Ernesto¹  Volpe, Pompeo¹  Biral, Donatella¹ 

[1] Centro di Studio per la Biologia e la Fisiopatologia Muscolare del CNR, Istituto di Patologia Generale dell'Università di Padova, via Trieste 75, 35121 Padova, Italy

关键词: Skeletal muscle;    Sarcoplasmic reticulum;    Calsequestrin;    Ca2+;   

DOI  :  10.1016/0014-5793(92)80241-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca²⁺ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297–300), and can be both purified from slow-twitch skeletal muscle of the rabbit (Damiani, E., Volpe, P. and Margreth, A. (1990) J. Muscle Res. Cell Motil. 11, 522–530). Here we show that both CS isoforms coexist in slow-twitch muscle fibers as indicated by indirect immunofluorescent staining of cryosections with affinity-purified antibodies specific for each CS isoform.

【 授权许可】

Unknown