Complex formation between calsequestrin and the ryanodine receptor in fast‐ and slow‐twitch rabbit skeletal muscle

FEBS Letters

Ohlendieck, Kay¹  Murray, Brendan E.¹ 

[1] Department of Pharmacology, University College Dublin, Belfield, Dublin 4, Ireland

关键词: Calsequestrin;    Ryanodine receptor;    Excitation-contraction coupling;    Triad;    Skeletal muscle;    CSQ;    calsequestrin;    DHPR;    dihydropyridine receptor;    DSP;    dithiobis-succinimidyl propionate;    EC;    excitation-contraction;    mAb;    monoclonal antibody;    RyR;    ryanodine receptor;    SR;    sarcoplasmic reticulum;   

DOI  :  10.1016/S0014-5793(98)00621-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Linkage between the high-capacity Ca²⁺-binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca²⁺-release during skeletal muscle excitation-contraction coupling. However, no direct biochemical evidence exists showing a connection between these high-molecular-mass complexes in native skeletal muscle membranes. Here, using immunoblot analysis of chemically crosslinked membrane vesicles enriched in triad junctions, we have demonstrated that a very close neighborhood relationship exists between calsequestrin and the ryanodine receptor in both main fiber types. Hence, the luminal Ca²⁺-reservoir complex appears to be directly coupled to the membrane Ca²⁺-release complex and oligomerization seems to be of functional importance.

【 授权许可】

Unknown