Intracellular delivery of protein kinase C‐α or ε isoform‐specific antibodies promotes acquisition of a morphologically differentiated phenotype in neuroblastoma cells

FEBS Letters

Leli, Ubaldo¹  Parker, Peter J.²  Shea, Thomas B.³ 

[1] Ralph Lowell Laboratories Harvard Medical School, Belmont, MA, USA;Imperial Cancer Research Fund, London, UK;Laboratory for Cellular & Developmental Neurobiology Harvard Medical School, Belmont, MA, USA

关键词: Differentiation;    Isoform;    Neuroblastoma;    Neuritogenesis;    Protein kinase;   

DOI  :  10.1016/0014-5793(92)80334-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protein kinase C (PKC) family participates in a ubiquitous cell signalling system utilizing increased turnover of phosphoinositides. Because down-regulation of total PKC activity has been implicated in the acquisition of a morphologically differentiated phenotype in SH-SY5Y neuroblastoma cells, we aimed to identify the specific PKC isoforms in this process. Here we report that intracellular delivery of PKC-α and -ε, but not -β_|4-γ or -δ isoform-specific antibodies is sufficient to induce acquisition of a morphologically differentiated phenotype in SH-SY5Y neuroblastoma cells.

【 授权许可】

Unknown