Expression analysis of ruminant α‐lactalbumin in transgenic mice: Developmental regulation and general location of important cis‐regulatory elements

FEBS Letters

Soulier, S.¹  Mercier, J.C.¹  Vilotte, J.L.¹  Stinnakre, M.G.¹ 

[1] Laboratoire de Génétique Biochimique, INRA-CRJ, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France

关键词: α-Lactalbumin;    Goat;    Bovine;    Transgenic mouse;    Tissue-specificity;    Milk composition;    αLa;    α-lactalbumin;    gαLa;    goat α-lactalbumin;    bαLa;    bovine α-lactalbumin;    WAP;    whey acidic protein;   

DOI  :  10.1016/0014-5793(92)80317-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The bovine α-lactalbumin transgene with 750 bp and 336 bp of the 5′ and 3′ flanking region, respectively, is developmentally regulated as its endogenous counterpart in transgenic mice. Comparative expression analysis of three 5′-shortened constructs suggests that the region −4771/−220 contains important cis-acting transcriptional elements. The level of expression of a long caprine α-lactalbumin transgene encompassing 8.5 kb and 9.5 kb of the 5′ and 3′ flanking region, respectively, was higher but still unrelated to the copy number. Expression of the transgenes and of endogenous milk-protein genes was tissue-specific. In contrast with a recent report, only low amounts of the relevant mRNA were detected in some skin samples, which suggests a possible contamination by mammary tissue.

【 授权许可】

Unknown