The bovine α‐lactalbumin promoter directs expression of ovine trophoblast interferon in the mammary gland of transgenic mice

FEBS Letters

Charlier, M.²  L'Haridon, R.³  Soulier, S.⁴  Gaye, P.¹  Mercier, J.C.⁴  Vilotte, J.L.⁴  Stinnakre, M.G.⁴ 

[1] Unité d'Endocrinologie de l'Embryon and Unité d'Endocrinologie Moléculaire, I.N.R.A., 78350 Jouy-en-Josas, France;Laboratoire de Virologie/Immunologie, I.N.R.A., 78350 Jouy-en-Josas, France;Laboratoire de Génétique Biochimique, I.N.R.A., 78350 Jouy-en-Josas, France;Laboratoire de Physiologie Comparée, Universit'e Paris VI, 2 place Jussieu, 75005 Paris, France

关键词: α-Lactalbumin;    Trophosblast interferon;    Bovine;    Ovine;    Transgenic mouse;    Milk composition;    bαLa;    bovine α-lactalbumin;    oTP;    ovine trophoblastin;    IFN;    interferon;   

DOI  :  10.1016/0014-5793(91)80751-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A hybrid construct derived from ovine trophoblastin cDNA and bovine α-lactalbumin-encoding gene, was injected into the pronuclei of mouse eggs. In one of the resulting transgenic mouse lines, expression of the hybrid construct was detected and found to be limited to the mammary gland of lactating females which secreted active ovine trophoblastin. This strongly suggests that important cis-acting DNA sequences involved in tissue-specific expression of the bovine gene are located within the second half of the 3′ untranslated region, or/and the proximal 5′and 3′ regions flanking the transcriptional unit.

【 授权许可】

Unknown