Epidermal growth factor (EGF) decreased endothelin‐2 (ET‐2) production in human renal adenocarcinoma cells

FEBS Letters

Hosoya, Masaki¹  Matsumoto, Hirokazu¹  Ohkubo, Shoichi¹  Suzuki, Nobuhiro¹  Tokito, Fumino¹  Fujino, Masahiko¹ 

[1] Tsukuba Research Laboratories, Takeda Chemical Industries Ltd., Wadai 10 Tsukuba, Ibaraki 300-42, Japan

关键词: Endothelin-2;    Epidermal growth factor;    Enzyme immunoassay;    ACHN cell;    ET-1;    ET-2 and ET-3;    endothelin-1;    -2 and -3;    EIA;    enzyme immunoassay;    bFGF;    basic fibroblast growth factor;    EGF;    epidermal growth factor;    IGF-I and IGF-II;    insulin-like growth factor-I and -II;    NGF;    nerve growth factor;    PDGF;    platelet-derived growth factor;    TGF-α and -β;    transforming growth factor-α and -β;   

DOI  :  10.1016/0014-5793(91)81374-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Production of immunoreactive (ir-) endothelin-2 (ET-2) in renal adenocarcinoma cells, ACHN, was reduced by transforming growth factor-β, basic fibroblast growth factor, transforming growth factor-α and, most strikingly, by epidermal growth factor (EGF). These growth factors did not show such inhibitory effects on the secretion of ir-ET-1 in ET-1-producing cells, indicating that the production of ET-2 and ET-1 is regulated differently by the growth factors. EGF specifically reduced the secretion of not only ir-ET-2 but also ir-big ET-2 with only a small decrease in total protein synthesis. Northern blot analysis indicated that EGF controls the ET-2-production at the transcription levels of ET-2 gene.

【 授权许可】

Unknown