Effect of phosphoramidon on big endothelin‐2 conversion into endothelin‐2 in human renal adenocarcinoma (ACHN) cells Analysis of endothelin‐2 biosynthetic pathway

FEBS Letters

Saito, Toshihiro³  Kimura, Sadao³  Nishiyama, Mariko⁴  Shinmi, Osamu⁴  Inagaki, Yoshiaki³  Masaki, Tomoh¹  Moroi, Kayoko⁴  Yorimitsu, Kazuyuki³  Sugita, Yoshiki² 

[1] Department of Pharmacology, Kyoto University School of Medicine, Kyoto 606, Japan;Department of Biochemistry, Institute of Basic Medical Sciences, Tsukuba University, Tsukuba 305, Japan;The Third Department of Internal Medicine and School of Medicine, Chiva University, Chiba 260, Japan;Center for Neurobiology and Molecular Immunology, School of Medicine, Chiva University, Chiba 260, Japan

关键词: Endothelin-2;    Big endothelin-2;    Endothelin-converting enzyme;    Phosphoramidon;    Human renal adenocarcinoma (ACHN) cell;    ET;    endothelin;    ECE;    endothelin-converting enzyme;    Trp;    tryptophan;    Val;    valine;    As-ETC;    anti-ET-2(15–21) antiserum;    As-bET2C;    anti-big ET-2(31–38) antiserum;    As-ETL;    anti-ET-2(1–21) antiserum;    AcCN;    acetonitril;    TFA;    trifluoroacetic acid;    HPLC;    high performance liquid chromatography;    RIA;    radioimmunoassay;    ir;    immunoreactive;   

DOI  :  10.1016/0014-5793(92)81513-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biosynthetic pathway of endothelin (ET)-2 was analyzed in cultured ACHN cells. In the supernatant, we detected three ET-2-related peptides, ET-2, big ET-2(1–38) and big ET-2(22–28). Phosphoramidon decreased the amount of ET-2 and increased that of big ET-2(13̄8)dose-dependently. The amount of big ET-2(1–37) did not significantly change. These results suggest that big ET-2 is composed of 38 and not 37 amino acid residues, and that a putative ET-2-converting enzyme (ECE-2) should be classified as a phosphoramidon-sensitive neutral metalloprotease, bearing a resemblance to the putative ET-1-converting enzyme (ECE-1) in endothelial cells.

【 授权许可】

Unknown