Phosphorylation of recoverin, the calcium‐sensitive activator of photoreceptor guanylyl cyclase

FEBS Letters

Koch, K.-W.¹  Lambrecht, H.-G.¹ 

[1] Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Postfach 1913, W-5170 Jülich, Germany

关键词: Photoreceptor;    Guanylyl cyclase;    Recoverin;    Phosphorylation;    Light adaptation;    ROS;    rod outer segments;    cGMP;    guanosine-3′;    5′-cyclic monophosphate;    GC;    guanylyl cyclase;    PDE;    cGMP phosphodiesterase;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    DTT;    dithiothreitol;    PMSH;    phenylmethyl-sulfonyl fluoride;    FPLC;    fast protein liquid chromatography;   

DOI  :  10.1016/0014-5793(91)80670-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recoverin, a new calcium binding protein from bovine rod photoreceptor cells, activates guanylyl cyclase below a free calcium concentration of 200 nM. We show here that recoverin is phosphorylated by an endogeneous kinase and Mg-ATP at the same decreased calcium concentration. The calcium-dependent activation of guanylyl cyclase is enhanced in the presence of ATP. We suggest that phosphorylation of recoverin reinforces the stimulation of guanylyl cyclase at decreased calcium concentrations.

【 授权许可】

Unknown