The gene organisation of the human β2 integrin subunit (CD18)

FEBS Letters

Wells, Charmian E.¹  Wright, Ann H.¹  Law, S.K.Alex¹  Weitzman, Jonathan B.¹  Clark, Paula A.¹ 

[1] The MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK

关键词: Integrin;    Gene structure;    Exon/intron boundary;    PCR;    polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;    PAGE;    polyacrylamide gel electrophoresis;    PMA;    phorbol myristate acetate;    RFLP;    restriction fragment length polymorphism;   

DOI  :  10.1016/0014-5793(91)81351-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the gene of the human β2 integrin subunit (CD18) and found it to be organised into 16 exons spanning a region of about 40 kb. All exon/intron boundaries conform to the GT/AG splicing consensus. The exons coding for the cysteine-rich region, which has been postulated to consist of 3 or 4 repeating elements, are not organised correspondingly. Transcription of the gene initiates from multiple sites which may be due to the absence of an upstream TATA box. The polyadenylation site is also heterogeneous. Five different sites were identified over a stretch of 10 bases.

【 授权许可】

Unknown