Structural characterization and expression of a brain specific gene encoding chick kainate binding protein

FEBS Letters

Wada, Keiji¹  Eshhar, Nomi¹  Hunter, Chyren¹  Wenthold, Robert J.¹ 

[1] Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Glutamate receptor;    Kainate;    Gene structure;    Promoter;    Transcription;    In situ hybridization;    c-KBP;    chick kainate-binding protein;    KBP;    kainate-binding protein;    GluR;    glutamate receptor;    PCR;    polymerase chain reaction;    nAChRs;    nicotinic acetylcholine receptor;   

DOI  :  10.1016/0014-5793(92)80551-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding chick kainate-binding protein (c-kbp), a memeber of the non-NMDA ionotropic glutamate receptor family has been isolated and characterized. The c-KBP gene is at least 13 kilobases long and contains 11 exons interrupted by 10 introns. Primer extension and RNase protection studies identified a major trranscription initiation site located 117 bases upstream from the initiation methionine codon ATG. Consensus TATA and CCAAT sequences were detected in the putative promoter region. The structure of the c-KBP gene is strikingly different from that of other members of neurotransmitter-gated ion-channels (cloned at present) although the topology of c-KBP consists of four membrane-spanning domains, a structural characteristics of ionotropic receptor subunits. The c-KBP gene was found to be expressed at high levels in chick cerebellar Bergmann glia and at extremely low levels in the forebrain. The limited expression of the c-KBP gene raises important questions concerning the mechanisms governing the regulation of c-KBP gene transcription.

【 授权许可】

Unknown