Overexpression of alcohol oxidase in Pichia pastoris

FEBS Letters

Keizer-Gunnink, I.²  Veenhuis, M.²  de Hoop, M.J.¹  Ab, G.¹  Sjollema, Klaas²  Cregg, J.³ 

[1] Laboratory of Biochemistry, University of Groningen, Nijenborgh 16, 9747 AG Groningen, The Netherlands;Laboratory for Electron Microscopy, University of Groningen, Kerklaan 30, 9751 NN Groningen, The Netherlands;Dept. of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 19600 Von Neumann Dr., Beaverton OR 97006-1999, USA

关键词: Alcohol oxidase;    Methylotrophic yeast;    Peroxisome;    Hp-AOX;    the alcohol oxidase gene from Hansenula polymorpha;   

DOI  :  10.1016/0014-5793(91)81306-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein. The excess remained in the cytosol as protein aggregates composed of monomers. These results are discussed in view of the possible application of peroxisomes as storage compartments for heterologous proteins.

【 授权许可】

Unknown