Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris

Bioresources and Bioprocessing

Xiaojun Ma¹  Lun Yao¹  Yunxia Li²  Xiaoxin Zhai²  Yongjin J. Zhou²  Lingyun Jia³  Peng Cai³ 

[1] Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences;Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences;School of Life Science and Biotechnology, Dalian University of Technology;

关键词: Methylotrophic yeast;    Pichia pastoris;    α-Alkenes;    Methanol biorefinery;    Cofactor engineering;    Peroxisome;   

DOI  :  10.1186/s40643-022-00551-1

来源: DOAJ

【 摘 要 】

Abstract α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals. Graphical Abstract

【 授权许可】

Unknown