Overproduction, purification and crystallization of Bacillus cereus oligo‐1,6‐glucosidase

FEBS Letters

Hata, Yasuo²  Suzuki, Yuzuru¹  Kitamura, Kazuhisa¹  Katsube, Yukiteru²  Watanabe, Kunihiko¹ 

[1] Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo, Kyoto 606, Japan;Institute for Protein Research, Osaka University, Yamada-oka, Suita, Osaka 565, Japan

关键词: Oligo-1;    6-glucosidase;    Proline theory;    Thermostabilization;    Crystallization;    X-Ray analysis: Overexpression;   

DOI  :  10.1016/0014-5793(91)81264-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene coding for oligo-1,6-glucosidase from Bacillus cereus ATCC7064 has been overexpressed in Escherichia coli MV1184 cells under the control of the lac promoter in the genetically engineered plasmid pBCE4-2. Oligo-1,6-glucosidase was purified in large quantities and was crystallized at 25°C by using a hanging drop vapor diffusion method with 53% saturated ammonium sulfate. The crystals have the shape of hexagonal bipyramids and belong to the space group P6₂ or P6₄ with lattice constants of a=b=106.1 Å, c=120.0 Å and γ=120°.

【 授权许可】

Unknown