Biochemical approaches of increasing thermostability of β‐amylase from Bacillus megaterium B6

FEBS Letters

Nanda, Geeta¹  Ray, Rina Rani¹  Jana, Subhas Chandra¹ 

[1]Department of Microbiology, Bose Institute, P-1/12, CIT Scheme VIIM, Calcutta-700 054, India

关键词: β-Amylase;    Thermostabilization;    Adsorption;    Immobilization;    Irreversible thermoinactivation;   

DOI  :  10.1016/0014-5793(94)01227-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Studies on the irreversible thermoinactivation of β-amylase from Bacillus megaterium B₆ exposed to 60°C revealed that the deactivation mechanism probably results from the oxidation of thiols present at the active site of the enzyme. Several attempts were made to increase its thermostability, which indicated that Mn²⁺ played a key role in determining thermostability and partially reactivating the inactivated enzyme. Immobilization of β-amylase through gel-entrapment and covalent crosslinking brought about a remarkable increase in thermotolerance with about a 14-fold increase in catalytic half-life.

【 授权许可】

Unknown