Effects of cholesterol‐ or 7‐ketocholesterol‐containing liposomes on colony‐forming ability of cultured cells

FEBS Letters

Ivanov, Aleksey S.¹  Prokhorov, Leonid Yu.²  Archakov, Alexander I.¹  Khokhlov, Alexander N.² 

[1] Institute of Biological and Medical Chemistry, Academy of Medical Sciences, Moscow, USSR;Evolutionary Cytogerontology Sector, Faculty of Biology, Moscow State University, Moscow, USSR

关键词: Cholesterol;    7-Ketocholesterol;    Liposome;    Cultured cell;    Colony-forming ability;   

DOI  :  10.1016/0014-5793(91)81252-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Experiments with cultured Chinese hamster cells showed that incubation of the cells with (phosphatidylcholine + cholesterol + 7-ketocholesterol)-containing liposomes (4:3:1 by weight) during two hours led to a decrease in the colony-forming ability of cells down to zero, while (phosphatidylcholine + cholesterol)-containing liposomes (1:1 by weight) reduce this parameter by 90%. Furthermore, the cholesterol-containing liposomes (without 7-ketocholesterol) induce a decrease in the number of the maximal-site colonies accompanied by the corresponding increase in the number of the middle-size colonies.

【 授权许可】

Unknown