Stimulation of phospholipase A2 activity by high osmotic pressure on cholesterol‐containing phospholipid vesicles

FEBS Letters

Utsumi, Kozo¹  Okimasu, Eiji²  Sasaki, Junzo² 

[1] Department of Medical Biology, Kochi Medical School, Kochi 781-51, Japan;Department of Anatomy, Okayama University Medical School, Okayama 700, Japan

关键词: Phospholipase A2;    Liposome;    Cholesterol;    Osmolarity;    Phase transition;    Bilayer curvature;   

DOI  :  10.1016/0014-5793(84)80203-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hydrolytic action of porcine pancreatic phospholipase A₂ (PLA₂) on cholesterol-containing dipalmitoylphosphatidylcholine liposomes was studied under various osmotic conditions by means of the phase transition release (PTR) technique. A reduced rate in the release of carboxyfluorescence (CF) from the cholesterol-containing liposomes was observed with treatment by PLA₂ concomitant with the decrease in its enzymatic activity. However, the reduced PLA₂ activity was elevated about 5-fold in medium of high osmotic pressure. This evidence suggests that high osmotic pressure might be responsible for the molecular packing at the bilayer surface or the high curvature of liposomal membrane structure.

【 授权许可】

Unknown