Primary structure of the cDNA 5′‐terminal region encoding the N‐terminal domain of the rabbit muscle α‐actinin subunit

FEBS Letters

Alakhov, Yu.B.²  Jokhadze, G.G.¹  Oleinikov, A.V.²  Zaalishvili, M.M.¹  Nadirashvili, N.Sh.¹ 

[1] Institute of Molecular Biology and Biological Physics, Georgian Academy of Sciences, 380060 Tbilisi, USSR;Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR

关键词: α-Actinin;    Rabbit skeletal muscle;    96-Fold degenerate oligonucleotide mix;    AMV reverse transcriptase strong stop signal;   

DOI  :  10.1016/0014-5793(91)81067-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A number of cDNA clones have been obtained in summary encoding the N-terminal domain containing 286 amino acid residues of the rabbit skeletal muscle α-actinin subunit. α-Actinin cDNA clones were isolated from specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. A strong stop signal for AMV reverse transcriptase in the 5′-terminal region of mRNA of α-actinin was found. It seems there is a G+C rich region (93% G+C nucleotides) including a continuous sequence of 23 G and C nucleotides encoding 6 glycine residues.

【 授权许可】

Unknown