期刊论文详细信息
FEBS Letters | |
Primary structure of the cDNA 5′‐terminal region encoding the N‐terminal domain of the rabbit muscle α‐actinin subunit | |
Alakhov, Yu.B.2  Jokhadze, G.G.1  Oleinikov, A.V.2  Zaalishvili, M.M.1  Nadirashvili, N.Sh.1  | |
[1] Institute of Molecular Biology and Biological Physics, Georgian Academy of Sciences, 380060 Tbilisi, USSR;Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR | |
关键词: α-Actinin; Rabbit skeletal muscle; 96-Fold degenerate oligonucleotide mix; AMV reverse transcriptase strong stop signal; | |
DOI : 10.1016/0014-5793(91)81067-I | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A number of cDNA clones have been obtained in summary encoding the N-terminal domain containing 286 amino acid residues of the rabbit skeletal muscle α-actinin subunit. α-Actinin cDNA clones were isolated from specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. A strong stop signal for AMV reverse transcriptase in the 5′-terminal region of mRNA of α-actinin was found. It seems there is a G+C rich region (93% G+C nucleotides) including a continuous sequence of 23 G and C nucleotides encoding 6 glycine residues.
【 授权许可】
Unknown
【 预 览 】
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