Electrophoretic separation of developmental and adult rabbit skeletal muscle myosin heavy chain isoforms: Example of application to muscle denervation study

FEBS Letters

d' Albis, Anne¹  Janmot, Chantal¹ 

[1] Laboratoire de Biologie Physicochimique, Bâtiment 433, Unité de Recherche Associée au CNRS no. 1131, Université Paris-Sud 91405, Orsay, France

关键词: Myosin heavy chain;    MHC;    SDS gel-electrophoresis;    Rabbit skeletal muscle;   

DOI  :  10.1016/0014-5793(94)01002-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We present the separation by SDS gel-electrophoresis of the six main myosin heavy chains (MHC) present in rabbit skeletal muscle. The separation of the four adult MHC (1, 2A, 2X/2D, 2B) was compared to that of the corresponding rat MHC as described by Talmadge and Roy [J. Appl. Physiol. 99 (1993) 2337-2340]. We found that many rabbit muscles contained mainly one of the four MHC, in some cases the 2B MHC. In addition, we resolved the embryonic E and perinatal P developmental MHC, which should facilitate muscle differentiation and regeneration studies in the rabbit. An example of application to the study of muscle denervation is given.

【 授权许可】

Unknown