Protein kinase C subtypes in endothelial cells

FEBS Letters

Mattila, Pirkko¹ 

[1] Department of Bacteriology and Immology, University of Helsinki, Haartmaninkatu 3, SF-00290 Helsinki, Finland

关键词: Endothelial cell;    Protein kinase C;    Interferon-gamma;    Diacylglycerol;   

DOI  :  10.1016/0014-5793(91)80914-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of protein kinase C (PKC) has been linked to the regulation of class II expression on endothelial cells by interferon-γ (IFN-γ). PKC subtypes in endothelial cells were analyzed using three different approaches, the immunoperoxidase staining of native and IFN-γ stimulated cells cultured on chamber slides as well as immuno- and Northern blotting. All approaches revealed that of the conventional subtypes. α is the predominant form of PKC in endothelial cells. Even though IFN-γ is able to induce PKC translocation to particulate fractions, no translocation was detected in histological stainings. Western blot studies as well as mRNA studies revealed that IFN-γ is unable to increase the total amount of PKC in endothelial cells.

【 授权许可】

Unknown