Suppression by staurosporine of Ca2+‐mobilization triggered by ligation of antigen‐specific receptors on T and B lymphocytes An essential role of protein tyrosine kinase in the signal transduction

FEBS Letters

Yamashita, Yumi¹  Hasegawa-Sasaki, Hiroko¹  Sasaki, Terukatsu¹ 

[1] Department of Biochemistry, Cancer Research Institute, Sapporo Medical College, Sapporo 060, Japan

关键词: T cell receptor;    Membrane immunoglobulin;    Staurosporine;    Calcium ion mobilization;    Platelet-derived growth factor;    Protein-tyrosine kinase;    PLC;    phospholipase C;    [Ca2+]i;    intracellular free Ca2+ concentration;    PGF2α;    prostaglandin F2α;    PTK;    protein-tyrosine kinase;    PDGF;    platelet-derived growth factor;    TCR;    T cell receptor;    mlg;    membrane immunoglobulin;    H-7;    1-(5-isoquinolinylsulfonyl)-2-methyl-piperazine;    H-8;    N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide;   

DOI  :  10.1016/0014-5793(91)81000-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is known that the receptor for platelet-derived growth factor (PDGF) activates phospholipase C (PLC) by phosphorylating the γl isoform of PLC with the receptor protein-tyrosine kinase (PTK), whereas a guanine nucleotide-binding protein participates as a transducer in the PLC activation through the receptors for vasopressin, bombesin and prostaglandin F_2α (PGF_2α). We have shown in a rat fibroblast line that staurosporine is a potent PTK inhibitor capable of clearly discriminating the two types of receptor-stimulated Ca²⁺ mobilization and, by inference, PLC activation: the response triggered by PDGF was completely inhibited, whereas the responses triggered by vasopressin, bombesin and PGF_2α were not affected at all. The Ca²⁺ mobilization in human T and B cell lines induced by anti-CD3 and anti-immunoglobulins (Ig) was completely suppressed by staurosporine. The results indicate that the PTK activity plays an essential role in the PLC activation through the T cell receptor/CD3 complex and through membrane Ig.

【 授权许可】

Unknown