FEBS Letters | |
[3H]Morphine binding is enhanced by IL‐1‐stimulated thymocyte proliferation | |
Ramakrishnan, S.1  Roy, Sabita1  Loh, Horace H.1  Lee, Nancy M.1  Ge, Bang-Lun1  | |
[1] Department of Pharmacology, 3-249 Millard Hall, 435 Delaware St., University of Minnesota Medical School, Minneapolis, MN 55455, USA | |
关键词: Immune; Interleukin; Opioid binding; Cell proliferation; Up-regulation; Phorbol ester; IL-1; interleukin-1; HL-2; interleukin-2; PHA; phytohemagglutinin; PMA; phorbol myristate acetate; | |
DOI : 10.1016/0014-5793(91)80023-V | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Mouse thymocytes incubated in vitro with increasing concentrations of interleukin-1 (IL-1) in the presence of phytohemagglutinin (PHA) exhibited a dose-dependent increase in cell proliferation, as measured by [3H]thymidine incorporation. Under these conditions, there was a parallel dosedependent increase in specific [3H]morphine binding, with a maximum increase of approximately 5-fold over basal levels. The binding sites differ from classical opioid receptors in that they are not stereo-selective. Interleukin-2 was ineffective in promoting either cell proliferation or enhanced opioid binding, but the effects of IL-1 could be mimicked by phorbol myristate acetate (PMA), suggesting the involvement of tyrosine phosphorylation. These results indicate that morphine-binding sites on immune cells can be regulated by cytokine activation.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020295157ZK.pdf | 355KB | download |