[3H]Morphine binding is enhanced by IL‐1‐stimulated thymocyte proliferation

FEBS Letters

Ramakrishnan, S.¹  Roy, Sabita¹  Loh, Horace H.¹  Lee, Nancy M.¹  Ge, Bang-Lun¹ 

[1] Department of Pharmacology, 3-249 Millard Hall, 435 Delaware St., University of Minnesota Medical School, Minneapolis, MN 55455, USA

关键词: Immune;    Interleukin;    Opioid binding;    Cell proliferation;    Up-regulation;    Phorbol ester;    IL-1;    interleukin-1;    HL-2;    interleukin-2;    PHA;    phytohemagglutinin;    PMA;    phorbol myristate acetate;   

DOI  :  10.1016/0014-5793(91)80023-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse thymocytes incubated in vitro with increasing concentrations of interleukin-1 (IL-1) in the presence of phytohemagglutinin (PHA) exhibited a dose-dependent increase in cell proliferation, as measured by [³H]thymidine incorporation. Under these conditions, there was a parallel dosedependent increase in specific [³H]morphine binding, with a maximum increase of approximately 5-fold over basal levels. The binding sites differ from classical opioid receptors in that they are not stereo-selective. Interleukin-2 was ineffective in promoting either cell proliferation or enhanced opioid binding, but the effects of IL-1 could be mimicked by phorbol myristate acetate (PMA), suggesting the involvement of tyrosine phosphorylation. These results indicate that morphine-binding sites on immune cells can be regulated by cytokine activation.

【 授权许可】

Unknown