p21ras contributes to HIV‐1 activation in T‐cells

FEBS Letters

Telford, John L.¹  Macchia, Giovanni¹  Massone, Annalisa¹  Baldari, Cosima T.² 

[1] I.R.I.S., Via Fiorentina 1, 53100 Siena, Italy;Department of Evolutionary Biology, University of Siena, Via Mattioli 4, 53100 Siena, Italy

关键词: HIV-1;    Interleukin;    Signal transduction;    Cyclosporin A;    LTR;    long terminal repeat;    PKC;    protein kinase C;    CAT;    acetyl chloramphenicol transferase;    CsA;    cyclosporin A;   

DOI  :  10.1016/0014-5793(92)80633-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of T-cells infected by HIV-1 results in activation of long terminal repeal (LTR)-dependent viral transcription and ultimately the production of infectious virus. Although fulI T-cell activation requires a complex series of intracellular signals, including protein kinase C activation, calcium mobilisation, and less-well defined lymphokine-induced signals, the HIV-1 LTR can be activated by subsets of these signals. We have studied the interaction of these signals in the human lymphoma line, Jurkat, in activation of the HIV-1 LTR. The HIV promoter was induced by IL-1 and phorbol ester activation of PKC but not by a calcium ionophore. The constitutively active form of Ha-ras could replace phorbol ester stimulation of the HIV promoter and of a synthetic promoter containing NFκB binding sites.

【 授权许可】

Unknown